Micropropagation for the Production of Underwater Grasses by Steve Ailstock and Deborah Shafer DEFINITION: Micropropagation is a technique that manipulates small quantities of axenic plant mate-rial, ranging from single cells to stem segments, under conditions favorable to the formation of new plants. A few creators include two additional stages (stage 0 and IV) for an increasingly extensive portrayal of micropropagation. The regeneration of a whole plant from single cell, tissue or small pieces of vegetative structures through tissue culture is called micropropagation. Micropropagation is the rapid vegetative propagation of plants under in vitro conditions of high light intensity, controlled temperature, and a defined nutrient medium. It has been predicted that by using large vessel labour cost can be reduced at least by 50% 1. Micropropagation Lab Report 837 Words | 4 Pages. 11 Advantages and Disadvantages of Cloning December 16, 2019 October 4, 2017 by Louise Gaille Cloning is a process that creates new life by copying the cell data of a living host. Advantages and Limitations of Micropropagation Advantages of Micropropagation: 1. Disadvantages Major basic Types of Micropropagation. Disadvantages of conventional methods ... Micropropagation. Stage 0: Since micropropagation should be conducted in a perfect sterile condition, it also involves a … Micropropagation is not always the perfect means of multiplying plants. Also, an almost infinite number of plants can be propagated from the initial explant. Disadvantages of micropropagation The disadvantages of micropropagation are as below: It involves a lot of skill and technical hands to perform micropropagation which is very expensive these days. Eswinia sp, Bacillus sp) contaminate and grow in cultures. An infected plant sample can produce infected progeny. Micropropagation will be employed to develop hybrids where incompatibility is a problem using embryo rescue. Sometimes plants or cultivars do not come true to type after being tissue cultured. Micropropagation is not always the perfect means of multiplying plants. Read more about this topic:  Micropropagation. 3. For this reason, many plant breeders do not utilize micropropagation because the cost is prohibitive. Disadvantages of tissue culture propagation Conditions that limits its use include: It is very expensive, and can have a labor cost of more than 70% A monoculture is produced after micropropagation, leading to a lack of overall disease resilience, as all progeny plants may be vulnerable to the same infections. Application of Micropropagation 3. For some developing countries, tissue culture is a way to grow higher yields of food - food that would’ve otherwise been difficult to grow or non-existent. Not all plants can be successfully tissue cultured, often because the proper medium for growth is not known or the plants produce secondary metabolic chemicals that stunt or kill the explant. Learn vocabulary, terms, and more with flashcards, games, and other study tools. • Micropropagation saves an enormous amount of care usually required by cuttings and seedlings (watering, weeding, spraying etc.) Micropropagation is a method of plant propagation using extremely small pieces of plant tissue taken from a carefully chosen and prepared mother plant, and growing these under laboratory conditions to produce new plants. Disadvantages of micropropagation There is a chance that the propagated plants will be less resilient to diseases due to the type of environment they are grown in. Micropropagation has the potential to help save endangered species. Disadvantages of Micropropagation: Micropropagation is not always the perfect method of propagation or multiplying plants or propagules. ADVERTISEMENTS: Read this article to learn about the techniques, factors, applications and disadvantages of micro propagation process and also about production of disease-free plants and its merits and demerits. Advantages. This is uncommon if the stock plants are carefully screened and vetted to prevent culturing plants infected wit… Get a lot of clones of plants with desired characteristics; Know what the plant is going to be; Less likely to get a disease as everything is sterile; Disadvantages. Commercialization and Economic Considerations. Apart from several promising advantages and benefits of micropropagation, some negative aspects are also attached to this technique. Micropropagation is not always the perfect means of multiplying plants. Mechanisation of the process could reduce labour costs, but has proven difficult to achieve, despite active attempts to develop technological solutions. Under favourable conditions cultures are prepared. 3. Advantages and Limitations of Micropropagation Advantages of Micropropagation: 1. The Advantages And Disadvantages Of Micropropagation Vs Traditional Propagation. Micropropagation needs very little space as compared to greenhouses. Micropropagation. Economic Considerations. Micropropagation can be used to generate in vitro mutants. It is veryexpensive, and can have a labor cost of more than 70% 2. Since micropropagation should be conducted in a perfect sterile condition, it also involves a lot of expenditure. 2.3.Disadvantages of Micropropagation Micropropagation is not always the perfect means of multiplying plants, conditions that limits its use include: Shoot multiplication […] Disadvantages Orchids and banana can be produced by the asexual method of reproduction only. Micro Propagation: Plants can be propagated by sexual (through generation of seeds) or asexual (through multiplication of vegetative parts) means. The microbial infection can be controlled by addition of antibiotics or fungicides. Culture contamination – by bacteria, fungi, viruses is the major problem in micropropagation. Micropropagation is a confusing procedure and essentially includes 3 phases (I, II, and III). Micropropagation can be carried out independent of seasons, hence can fetch higher market price being in out of season. Micropropagation- Stages, Types, Applications, Advantages, Limitations. Micropropagation may have started off its stint on the cultivation stage with a modicum of disdain and distrust, but it is now embedded in most industries, especially that of cannabis. For the large-scale micropropagation the use of small culture vessel (volume < 0.5 L) has a number of disadvantages, for instance, it can enhance the labour cost significantly. 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